Application

Research CategoryApoptosis & Cancer

Research Sub CategoryBCL2 & Inhibition

This Anti-Bax Antibody is validated for use in WB, IH(P) for the detection of Bax.

Immunohistochemistry (ABC method) on formalin-fixed paraffin-embedded sections (1:600-1:1500)

Immunoblot (1;2500) using ECL detection.

High levels of Bax-alpha are found in areas of the brain, including the cerebrum and cerebellum, as well as the small intestine, stomach, overy, testis, uterus, thymus, and spleen.

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

A synthetic peptide corresponding to amino acids 43-61 of the mouse Bax-alpha protein.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-434

Physical form

Liquid antiserum, no preservatives.

Specificity

Recognizes Mouse and Rat Bax-alpha, a 21 kDa protein, as well as a longer 24 kDa. Bax-beta isoform and a 5 kDa Bax-gamma isoform, which are present in much smaller amounts in most tissues. Does not react with other members of the Bcl-2 family, as verified by immunoblot.

Storage and Stability

Store at -20°C for up to 12 months in undiluted aliquots. Avoid repeated freeze-thaw cycles.